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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Targeting HMGB1-NFκb Axis and miR-21 by Glycyrrhizin: Role in Amelioration of Corneal Injury in a Mouse Model of Alkali Burn
doi: 10.3389/fphar.2022.841267
Figure Lengend Snippet: GLY reduces miR-21 expression in keratocytes, and blockade of miR-21 preventes keratocytes differentiation to myofibroblasts. (A) qRT-PCR shows significantly increased miR-21 level in HK stimulated by TNFα or TGF-β1. (B) mRNA expression levels of miR-21 and HMGB1 in HK treated by TNFα with or without GLY. (C) The expression level of miR-21 in HK after miR-21 agomir transfection. (D–E) Western blot and qRT-PCR were used to examine α-SMA expression after miR-21 transfection. HK were transfected with miR-21 agomir, antagomir, and their negative controls (NC) before treated with TGF-β1. Western blotting shows significantly increased of α-SMA in HK overexpressed miR-21 and the quantified data shown in (E) mRNA expression levels of α-SMA in HK (F) Data were analyzed using one-way ANOVA followed by Bonferroni’s multiple comparison test ( n = 3/group). The results are expressed as the means ± SD of at least three independent experiments, and qRT-PCR was conducted in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet:
Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Comparison